Mass spectrometry ms is an analytical technique which identifies compounds based on the atomic sample composition of the molecules and their charge state 1. Page 1 nptel video course nptel video course proteomics proteomics prof. These lecture notes were first drafted in dutch by peter van galen in 1992, when the only mass spectrometer in the faculty of science was the departments. This lecture 1 include pesticides classification, introduction to mass spectrometry, vacuum system for agilent gc ms ms and ab sciex lc ms ms. Lecture 1 introduction, history, scope and terminology of. Ms in drug discovery and pharmacokinetics lingjun li, university of wisconsinmadison bme 595chem 590 july 8, 2011 tsinghua university, beijing, china what is drug discovery. Mass spectroscopy minnesota state university moorhead. Still the sensitivity is quite good and can allow the identification of proteins from. Mass spectrometry is an analytical technique to measure the mass tocharge ratio of ions that can be used to identify unknown compounds, determine the isotopic composition of elements in a compound, and quantify the amount of a compound in a sample. The trajectories of lowmass ions in a qit were shown to be. It is used for determining masses of particles, for determining the elemental composition of a sample or molecule. Fundamentals of mass spectrometry notes, engineering, semester notes edurev is made by best teachers of. He observes negative ions, multiply charged ions and identifies isotopes of 20ne and 22ne. Mass spectrometry is the study of matter through the formation of gasphase ions that are detected and characterized by their mass and charge.
May 23, 2015 page 1 nptel video course nptel video course proteomics proteomics prof. Mass spectrometry 30 references 31 3 mass spectrometry 33 3. Mass spectrometry is an analytical technique to measure the masstocharge ratio of ions that can be used to identify unknown compounds, determine the isotopic composition of elements in a compound, and quantify the amount of a compound in a sample. Mass spectrometry lecture 1 1 mass spectrometry lecture 1.
Thomson constructs the first mass spectrometerand sees spectra of o 2, n 2, co, co 2 and cocl 2. Definitions of terms relating to mass spectrometry iupac. Steps in atomic mass spectrometry atomization conversion of substantial fraction of atoms formed into ions separating ions formed based on their mass tocharge mz ratio counting the ions produced of a given mz with some sort of transducer i. Eggers elaborate the plan for a timeofflight mass spectrometer after a principle proposed by w. In the past 30 years, mass spectrometry ms has undergone a spectacular devel opment, in terms of. Lecture 3 mass spectrometry principles of mass spectrometry mass spectrometry separates molecules based on the mass to charge ratio.
By convention, m is the numerical value for the mass of the ion and z is the numerical value for the charge of the ion. When non mass spectrometrists are talking about mass spectrometry it rather often sounds as if they were telling a story out of poes tales of mystery and imagination. Fundamentals of proteinpeptide analyses by mass spectrometry. Analyzing biomolecular interactions by mass spectrometry, first edition. Indeed, mass spectrometry appears to be regarded as a mysterious method, just good enough to supply some molecular weight information. The mass resolution of analyzers may not always be adequate to distinguish individual peaks. Goldstein discovers anode rays in a gas discharge tube. The mass spectrum of an aldehyde shows a parent peak at mz 58 and a base peak at mz 29. Data system mass detector analyzer vacuum system source inlet region more precisely mass spectrometry determines the mass of a molecule.
Lecture 1 introduction, history, scope and terminology of mass spectrometry. Nov 30, 2011 this video is part of a 28 lecture graduatelevel course titled organic spectroscopy taught at uc irvine by professor james s. Introduction mass spectrometry ms is an analytical technique that measures the mass tocharge particles. Noncovalent or native mass spectrometry we can ionize intact protein complexes using esi what can we learn. The common components of a modern icpms are shown in. Using the fragmentation patterns for acetone as a guide, predict the signals that you would find in the mass spectra of a 2butanone. Hplc cont the term reverse phase chromatographywas used because rp is a form of partition chromatography where chemically bonded phase is hydrophobic or nonpolar e. History of mass spectrometry 1942 first commercial sector mass spectrometer by cec.
Mass spectrometry involves the separation of charged species which are produced by a variety of ionisation methods in lcms. Since it takes 100 kcal of energy to cleave a typical s bond, 1600 kcal is an enormous amount of energy to come into. When nonmass spectrometrists are talking about mass spectrometry it rather often sounds as if they were telling a story out of poes tales of mystery and imagination. This is reversed from normal phase chromatography, where the. When ionization occurs most the ions do not even enter the mass spectrometer. Instead, mass spectrometry is based upon the motion of a charged particle, called an ion, in an electric or magnetic field. Mass spectrometry is a destructive method used to measure molecular weight and provide.
Hello everybody, welcome to week three of the coursera course and experimental methods in systems biology. Mass spectrometry dont call it spectroscopy absorption of electromagnetic radiation electron impact mass spectrometry 70 ev 1614 kcalmol contrast with energy from ir 110 kcalmol or nmr 0. This document is highly rated by students and has been viewed 503 times. Mass spectrometry and proteomics lecture 5 matthias trost newcastle university matthias. These isotopic distributions are calculated at a mz resolution of 10,000 555. While the icpaes is interfaced with an optical grating system, the plasma in an icpms instrument must enter into a vacuum so that atomic cations can be separated by a mass filter. For the analytical chemist, a mass spectrum is useful for two applications. It is critical that you develop a working knowledge of how each of these techniques provides structural information about organic molecules.
From the time of the second edition published in 2001 until now, much progress has been achieved. Lecture notes organic structure determination chemistry. Metabolomics introduction to metabolomics and metabolomes and analytical challenges. Parameters for noncovalent mass spectrometry generally done with tof instruments direct nanospray infusion out of volatile buffers typically ammonium acetate. Bioanalytical instrumentation mass spectrometry lecture 18. Relaxing studying music, brain power, focus concentration music. Predict some signals that you would expect to see in a mass spectrum of 2chloropropane. Lecture 1 fundamentals of protein chemistry and mass spectrometry. Therefore, blind analysis of unknown samples is possible since ms does not require detailed prior knowledge. Mass spectrometry for biophysics and structural biology native electrospray mass spectrometry native ion. The mass to charge ratio mz of the ion effects this motion. The mass to charge ratio mz is used to describe ions observed in mass spectrometry. Where available, lecture notes or unit study guides are included for the following sessions and topics in the table below. The separation chamber of the mass spectrometer is keep under a.
Esi is usually coupled in separation systems which are fed with slow ions. There are five general types of mass analyzers that can be used for the separation of ions in a mass spectrometry. Since the charge of an electron is known, the mass to charge ratio a measurement of an ions mass. In a mass spectrometer, a molecule is vaporized and ionized by bombardment with a beam of highenergy electrons 1600 kcal. Mass spectrometry has spread by discipline as illustrated. Mass spectroscopy mass spectroscopy is a technique causing the formation of the gaseous ions with or without fragmentation. Indeed, mass spectrometry appears to be regarded as a mysterious method, just good enough to. Modern methods used in structure determination of organic molecules. This video is part of a 28lecture graduatelevel course titled organic spectroscopy taught at uc irvine by professor james s. Yellow brick cinema relaxing music recommended for you. Interactions between an aro2 postdischarge and biphenyl.
Thomson scanned the magnetic field and used electrical detection to record the first mass spectra of which a 1912 example is shown. Weight 200,000 15,000 ei fab tsp 1,000 non polar apci highly polar esi. Monoisotopic mass is typically expressed in unified atomic. It is important to be aware of the capabilities of the mass analyzer one is using. Akz where z mass number protons neutrons and a atomic number protons. This is the first of a pair of videos intended to help senior school chemistry students with mass spectrometry. The mass spectrometer is an instrument designed to separate gas phase ions according to their mz mass to charge ratio value. Mass spectrometry based proteomics in the life sciences. Mass spectrometric methods for analyzing glycoproteins and glycans mass spectrometry in an omics world me. Stoichiometry of complex relative affinity and topology rates of subunit exchange assembly pathways.
Lecture 1 basics of mass spectrometry 1 mass spectrometry. Ppt mass spectrometry lecture 1 powerpoint presentation. Mass spectrometry and proteomics lecture 1 newcastle university. Find materials for this course in the pages linked along the left. Mass spectroscopy ppt free download as powerpoint presentation. This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. A mass spectrum plural spectra is a plot of the ion signal as a function of the masstocharge ratio. Lecture notes in eimass spectrometry by torben lund.
A general introduction to mass spectrometry, including the different types of ionization sources and mass analyzers typically used in stateoftheart biological, pharmaceutical, and environmental mass spectrometry. Mass spectrometry introduction department of chemistry. The separation chamber of the mass spectrometer is keep under a high vacuum and so all. The number of hits may also be reduced by taking an accurately measured isotope pattern of the ion into consideration. Anatomy of an irms lecture 6 mass analyzers 1 assigned reading. The process of creating a chemical compound with chemical, biological, pharmaceutical, pharmacokinetic and.
How do you separate ions of different mass, so that you can detectquantify them. Chapter 2 fragmentation and interpretation of spectra 2. This is a lecture and the second video takes you through past paper questions. Steps in atomic mass spectrometry atomization conversion of substantial fraction of atoms formed into ions separating ions formed based on their masstocharge mz ratio counting the ions produced of a given mz with some sort of transducer i. Ea elemental analysis ms mass spectrometry ir infrared spectroscopy nmr nuclear magnetic resonance. The ionized molecule often fragments into smaller ionsradicals. Mass spectrometry ms is an analytical technique that measures the mass tocharge ratio of charged particles. The recommendations in this document pertain to fundamental, technical, and experimental aspects of ion formation, mass selection, detection, and analysis of mass. Thomson discovers the electron and determines its mz nobel prize in 1906 1912 j. Organic lecture series 3 mass spectrometry ms organic lecture series 4 a mass spectrometer a mass spectrometer is designed to do three things convert neutral atoms or molecules into a beam of positive or negative ions separate the ions on the basis of their masstocharge mz ratio measure the relative abundance of each ion. Marcuskassel rrkm theory to fit the conditions of mass spectrometry and it. Gas chromatography working principle and instrumentation lecture gas chromatography lecture this chromatography lecture explains about the instrumentation, principle of gas chromatography. Chemistry 344 spectroscopy and spectrometry uwmadison.
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